曹毅

职务:

●微生物与代谢工程四川省重点实验室主任

●四川省生物信息与代谢工程共享实验平台主任

●中国微生物学会基础微生物委员会理事

●中国微生物学会资源微生物委员会理事

●四川省科技青年联合会理事

●教育部新世纪优秀人才

●四川省学术及技术带头人

●国家科技部、国家自然科学基金委、四川省科技厅、江苏省科技厅、天津市科技厅、广东省科技厅、成都市科技局项目评审专家

办公室：85412842传真：85412842

E-mail: cyi@scu.edu.cn，caoyi_01@163.com

学位简历

1998年6月甘肃农业大学获博士学位

工作简历

1993年四川大学生命科学学院 讲师

1995年四川大学生命科学学院 副教授

2002年四川大学生命科学学院 教授

2005年教育部新世纪优秀人才，四川省杰出青年学科带头人和四川省学术及技术带头人

2006年四川省生物信息与代谢工程共享实验平台主任

2009年微生物与代谢工程四川省重点实验室主任

2010年国家科技部农业预备项目评审专家

研究方向及内容，主要科研项目，主要学术成果：

主要从事微生物资源与代谢工程、微生物生物能源、功能基因组与生物信息、畜用生物制剂与生物安全等领域的研究工作。先后主持科技部国家973、国家支撑计划、基础性工作专项、国际合作项目，国家自然科学基金，教育部重点项目，四川省支撑计划、四川省杰出青年基金及成都市科技攻关项目等研究工作。

申请和获得11项发明专利，出版教材1部，编写国家微生物资源平台描述规范等。建立了四川省微生物资源保藏中心、四川省生物信息与代谢工程共享实验平台和微生物与代谢工程四川省重点实验室。

发表文章

1. Zhou H, Li X, Guo M, Xu Q, Cao Y, Qiao D, Cao Y, Xu H., Secretory Expression and Characterization of an Acidic Endo-Polygalacturonase Gene from Aspergillus niger SC323 in Saccharomyces cerevisiae. J Microbiol Biotechnol. 2015; 25(7):999-1006.

2. Jiafu Lin, Bohan Zhao, Yu Cao, Hui Xu, Shuhan Ma, Mingyue Guo, Dairong Qiao, Yi Cao, Rationally designed Fe-MCM-41 by protein size to enhance lipaseimmobilization, catalytic efficiency and performance, Applied Catalysis A: General, 2014, 478: 175–185

3. Sun X, Cao Y, Xu H, Liu Y, Sun J, Qiao D, Cao Y. Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process. Bioresour Technol. 2014;155:204-12.

4. Song T, Cao Y, Xu H, Zhang W, Fei B, Qiao D, Cao Y. Purification and characterization of a novel β-agarase of Paenibacillus sp. SSG-1 isolated from soil. J Biosci Bioeng. 2014;118(2):125-9.

5. Zhang S, Cao Y, Xie L, Qiao D, Cao Y. Effect of superoxide dismutase‑entrapped liposomes and protein transduction domain‑superoxide dismutase on human umbilical vein endothelial cells. Mol Med Rep. 2014; 9(4):1427-33.

6. Li XR, Xu H, Xie J, Yi QF, Li W, Qiao DR, Cao Y, Cao Y. Thermostable sites and catalytic characterization of xylanase XYNB of Aspergillus niger SCTCC 400264. J Microbiol Biotechnol. 2014; 24(4): 483-8.

7. Cao Y, Xu H, Xie L, Yi Y, Yu Y, Feng S, Qiao D, Cao Y.Fluorimetric analysis of the binding characteristics of 5-enolpyruvylshikimate-3-phosphate synthase with substrates in Dunaliella salina. J Basic Microbiol. 2014;54(9):937-44.

8. Zhang S, Li XR, Xu H, Cao Y, Ma SH, Cao Y, Qiao D. Molecular cloning and functional characterization of MnSOD from Dunaliella salina. J Basic Microbiol. 2014 May;54(5):438-47.

9. Yi Liu, Yuesong Xu, Ying Peng Yu, Rongqing Yu, Pei Li, Dairong Qiao, Yi Cao and Yu Cao, The biodiversity of oleaginous microalgae in Northern Qinghai-Tibet Plateau, African Journal of Microbioology research, 2014, 8(1): 66-74

10. Xinran Li, Weijia Zhang, Xi Li, Qian Wu, Hui Xu, Dairong Qiao, Yi Cao and Yu Cao, Construction of Fusion Protein XYNAB and Improvement of its pH and Thermal Stability from Aspergillus niger 400264, Journal of Pure and Applied Microbiology, 2014, 8(4): 2535-2542

11. Jianrui Sun, Yan Liu, Baimei Zhang, Mingyue Guo, Yi Cao and Dairong Qiao, The High Additional Value Products and Antimicrobial Activity of Chlorella protothecoides, Journal of Pure and Applied Microbiology, 22014,8(3): 1857-1865

12. Jianrui Sun, Qian Wu, Huoxiang Zhou, Hui Xu, Yu Cao, Li Xie, Yi Cao and Dairong Qiao, Correlation Between Polysaccharide Biosynthesis and Glycometabolism Related Enzymes of Chlamydomonas sp. YB-204 in Different Conditions, 2014,8(3): 1923-1928

13. Xin Lv, Weihua Zhu and Yi Cao, Screening, Identification, Cloning, Expression and Characterization of a-amylase from Bacillus amyloliquefaciens, Journal of Pure and Applied Microbiology, 2014, 8(2): 909-915

14. Liu Y, Yang Y, Zhao F, Fan X, Zhong W, Qiao D, Cao Y. Multi-drug resistant gram-negative enteric bacteria isolated from flies at Chengdu Airport, China. Southeast Asian J Trop Med Public Health. 2013;44(6):988-96.

15. Miao YZ, Xu H, Fei BJ, Qiao DR, Cao Y. PCR-RFLP analysis of the diversity of phytate-degrading bacteria in the Tibetan Plateau.Can J Microbiol. 2013;59(4):245-51.

16. Fei B, Xu H, Cao Y, Ma S, Guo H, Song T, Qiao D, Cao Y. multi-factors rational design strategy for enhancing the thermostability of Escherichia coli AppA phytase. J Ind Microbiol Biotechnol. 2013;40(5):457-64.

17. Fei B, Xu H, Zhang F, Li X, Ma S, Cao Y, Xie J, Qiao D, Cao Y. Relationship between Escherichia coli AppA phytase's thermostability and salt bridges. J Biosci Bioeng. 2013; 115(6): 623-7.

18. Fei B, Cao Y, Xu H, Li X, Song T, Fei Z, Qiao D, Cao Y. AppA C-terminal plays an important role in its thermostability in Escherichia coli. Curr Microbiol. 2013; 66(4):374-8.

19. Li SL, Lin Q, Li XR, Xu H, Yang YX, Qiao DR, Cao Y. Biodiversity of the oleaginous microorganisms in Tibetan Plateau. Braz J Microbiol. 2012; 43(2):627-34.

20. Jing Li, Shijie Chen, Lin Li, Yingpeng Yu, Zhongan Fei, Dairong Qiao and Yi Cao, Approach of directed evolution of microbial lipases and biodiesel catalysis，African Journal of Microbiology Research, 2012, 6(3): 637-642

20. Nan Jin, Shuhan Ma, Yan Liu, Xiuli Yi, Rong He, Hui Xu, Dai Rong Qiao and Yi Cao, Thermophilic xylanase production by Aspergillus niger in solid state fermentation using wheat straw and corn cob, African Journal of Microbiology Research, 2012, 6(2): 2387-2394

21. Weihua Zhu, Yu Cao, Wei Li, Bomiao Cui, Qiaofu Yi, Qin Zhao, Hui Xu, Dairong Qiao and Yi Cao, The Error-prone PCR of a-amylase from Bacillus amyloliquefaciens Toward Enhanced Acid Tolerance and Higher Specific Activity, Journal of Pure and Applied Microbiology, 2013, 7(3): 1489-1496

22. Wang L, Ye LJ, Pan Y, Cao Y. Two plate-based colorimetric assays for screening α-amino acid ester hydrolase with high synthesis/hydrolysis ratio. Enzyme Microb Technol. 2012; 51(2): 107-12.

23. Ye LJ, Wang L, Pan Y, Cao Y. Changing the specificity of α-amino acid ester hydrolase toward para-hydroxyl cephalosporins synthesis by site-directed saturation mutagenesis. Biotechnol Lett. 2012; 34(9): 1719-24.

24. Li SL, Lin Q, Li XR, Xu H, Yang YX, Qiao DR, Cao Y. Biodiversity of the oleaginous microorganisms in Tibetan Plateau. Braz J Microbiol. 2012; 43(2): 627-34.

25. Jia Fu Lin, Qiang Lin, Jing Li, Zhong An Fei, Xin Ran Li, Hui Xu, Dai Rong Qiao and Yi Cao, Bacterial diversity of lipase-producing strains in different soils in southwest of China and characteristics of lipase, African Journal of Microbiology Research, 2012, 6(16): 3797-3806

26. Hui Xu, Shuhan Ma, Yu Cao, Shunli Feng, Qingrui Xu, Dairong Qiao and Yi Cao, Light intensity dependent expression of Lhca gene family encoding LHCI in Dunaliella salina, African Journal of Microbiology Research, 2012, 6(32): 6165-6171

26. Yu-Zhi Miao, Qiang Lin, Yu Cao, Gui-Hua He, Dai-Rong Qiao, Yi Cao，xtraction of water-soluble polysaccharides (WSPS) from Chinese truffle and its application in frozen yogurt, Carbohydrate Polymers, 2011, 86: 566-573.

27. Xie J, Song L, Li X, Yi X, Xu H, Li J, Qiao D, Cao Y.Site-directed mutagenesis and thermostability of xylanase XYNB from Aspergillus niger 400264. Curr Microbiol. 2011, 62(1): 242-8.

28. Guihong Lan, Zengting Li, Hui zhang, Changjun Zou, Dairong Qiao and Yi Cao, Relationship of microbial diversity from recycled injection water and high-temperature petroleum reservoirs analyzed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE)，African，Journal of Biotechnology, 2011, 10(53): 11004-11010

29. Guihong Lan, Zengting Li, Hui zhang, Changjun Zou, Dairong Qiao and Yi Cao, Enrichment and diversity analysis of the thermophilic microbes in a high temperature petroleum reservoir，African Journal of Microbiology Research, 2011, 5(14): 1850-1857

30. Shi Lin Li, Shun Li Feng, Zeng Ting Li, Hui Xu, Ying Peng Yu, Dai Rong Qiao and Yi Cao, Isolation, identification and characterization of oleaginous fungi from the soil of Qinghai Plateau that utilize D-xylose，African，Journal of Microbiology Research, 2011, 5(15): 2075-2081.

31. Rui Zhao, Yu Cao, Hui Xu, Linfeng Lv, Dairong Qiao, Yi Cao, Analysis of Expressed Sequence Tags From The Green Alga Dunaliella Salina (Chlorophyta)，Journal of Phycology，2011, 152（2）：139-147

26. Zhang F, Xu H, Cao Y, Wen T, Lin J, Ma G, Qiao D, Cao Y. Role of Lys281 in the Dunaliella salina (6-4) photolyase reaction. Curr Microbiol. 2011; 62(1): 146-51.

32. Cao Y, Jin N, Xu H, Liu Y, Zhu WH, Li XR, Qiao DR, Cao Y. Characterization and alternative splicing of the complex I 19-kD subunit in Dunaliella salina: expression and mutual correlation of splice variants under diverse stresses. Biosci Biotechnol Biochem. 2010; 74(5):1073-8.

33. W. M. Chen, N. Jin, Y. Shi, Y. Q. Su, B. J. Fei, W. Li, D. R. Qiao and Y. Cao, Coordinate expression of light-harvesting chlorophyll a/b gene family of photosystem II and chlorophyll a oxygenase gene regulated by salt-induced phosphorylation in Dunaliella salina, 2010, 48(3):355-360

34. Jing Li, Shu Zhang, Guanghua Liu, Rui Zhao, Hui Xu, Dairong Qiao and Yi Cao, Separation and identification of lipase – producing strains and immobilized transesterification efficiency，African Journal of Microbiology Research, 2010, 4(6): 448-453

35. Cao Y, Yu RQ, Liu Y, Zhou HX, Song LL, Cao Y, Qiao DR Design, recombinant expression, and antibacterial activity of the cecropins-melittin hybrid antimicrobial peptides.Curr Microbiol. 2010; 61(3):169-75.

36. Zhu W, Qiao D, Huang M, Yang G, Xu H, Cao Y.Modifying thermostability of appA from Escherichia coli. Curr Microbiol. 2010;61(4):267-73.

37. Liu Q, Jiang C, Xu Z, Xu H, Zhao R, Qiao D, Cao Y. Analysis of protein interaction network and function of Staphylococcus aureus].Wei Sheng Wu Xue Bao. 2009;49(1):56-63.

38. Zhang F, Qiao D, Xu H, Liao C, Li S, Cao Y.Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis. J Microbiol. 2009;47(3):351-7.

39. Liu Y, Qiao DR, Zheng HB, Dai XL, Bai LH, Zeng J, Cao Y. Cloning and sequence analysis of the gene encoding 19-kD subunit of Complex I from Dunaliella salina. Mol Biol Rep. 2008; 35(3): 397-403.

40. Chen C, Bai LH, Qiao DR, Xu H, Dong GL, Ruan K, Huang F, Cao Y.Cloning and expression study of a putative carotene biosynthesis related (cbr) gene from the halotolerant green alga Dunaliella salina. Mol Biol Rep. 2008;35(3):321-7.

41. Liang X, Qiao D, Huang M, Yi X, Bai L, Xu H, Wei L, Zeng J, Cao Y. Identification of a gene encoding the light-harvesting chlorophyll a/b proteins of photosystem I in green alga Dunaliella salina. DNA Seq. 2008; 19(2):137-45.

42. Yan Lv X, Rong Qiao D, Xiong Y, Xu H, You FF, Cao Y, He X, Cao Y. Photoreactivation of (6-4) photolyase in Dunaliella salina. FEMS Microbiol Lett. 2008;283(1):42-6.

43. Yan-Li Du, Yu Cao, Fang Lu, Fang Li, Yi Cao, Xiu-Li Wang, Yu-Zhong Wang, Biodegradation behaviors of thermoplastic starch (TPS) and thermoplastic dialdehyde starch (TPDAS) under controlled composting conditions, Polymer Testing, 2008, 27(8):924-930

44. He Q, Qiao D, Bai L, Zhang Q, Yang W, Li Q, Cao Y. Cloning and characterization of a plastidic glycerol 3-phosphate dehydrogenase cDNA from Dunaliella salina. J Plant Physiol. 2007; 164(2): 214-20.

45. Yi Y, Qiao D, Bai L, Xu H, Li Y, Wang X, Cao Y.Cloning, expression, and functional characterization of the Dunaliella salina 5-enolpyruvylshikimate-3-phosphate synthase gene in Escherichia coli. J Microbiol. 2007; 45(2):153-7.

46. Cheng L, Qiao DR, Lu XY, Xiong Y, Bai LH, Xu H, Yang Y, Cao Y. Identification and expression of the gene product encoding a CPD photolyase from Dunaliella salina. J Photochem Photobiol B. 2007; 87(2): 137-43.

47. Yang W, Cao Y, Sun X, Huang F, He Q, Qiao D, Bai L.Isolation of a FAD-GPDH gene encoding a mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase from Dunaliella salina. J Basic Microbiol. 2007; 47(3): 266-74.

48. Gaopeng Lei, Dairong Qiao, Linhan Bai, Hui Xu and Yi Cao, Isolation and characterization of a mitogen-activated protein kinase gene in the halotolerant alga Dunaliella salina, 2007, 20(1):13-17

49. Liang Wei, Yi Cao, Linhan Bai, Xue Liang, Tingting Deng, Jing Li, Dairong Qiao. Cloning and expression of a gene coding for the major light-harvesting chlorophyll a/b protein of photosystem II in the green alga Dunaliella salina. Journal of Applied Phycology, 2007, 19: 89-94.

50. Yi Y, Yi C, Qian L, Min L, Long C, Linhan B, Zhirong Y, Dairong Q.Cloning and sequence analysis of the gene encoding (6-4)photolyase from Dunaliella salina. Biotechnol Lett. 2006; 28(5): 309-14.

51. Wei L, Cao Y, Liang X, Liu Y, Deng T, Bai L, Qiao D.Identification of two genes encoding the major light-harvesting chlorophyll a/b proteins of photosystem II in green alga Dunaliella salina. DNA Seq. 2006;17(5):370-7.

52. Qinghua H, Dairong Q, Qinglian Z, Shunji H, Yin L, Linhan B, Zhirong Y, Yi C.Cloning and expression studies of the Dunaliella salina UDP-glucose dehydrogenase cDNA.DNA Seq. 2005; 16(3): 202-6.

53. Qinghua He; Dairong Qiao; Qinglian Zhang; Yin Li; Hui Xu1; Liang Wei; Ying Gu; Yi Cao, Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina ,Journal of Applied Phycology, 2004 , 16(5):395-400

54. Liu Y, Jiang Y, Qiao DR, Cao Y. The mechanism and application of posttransrciptional gene silencing. Sheng Wu Gong Cheng Xue Bao. 2002; 18(2):140-3.