冯红 博士 教授

[电子邮件:hfeng@scu.edu.cn, 电话: 028-85412738 (Lab.); 手机: 13980772773]

简 历

四川农业大学，学士（1985.07）；四川农业大学，硕士(1988.07)；四川大学，博士(2000.07)。

1988.07-1997.09— 四川农业大学，副教授(1996)；

2000.07-2001.12— 成都百奥生物信息科技有限公司，研究发展部经理；

2001.12-2004.08 —美国Clemson University遗传与生物化学系博士后；

2004.12-至今—四川大学生命科学学院任教，教授(2007)。

研究方向:

•分子遗传学

•酶的生物化学与蛋白质工程

•资源生物转录组组学

研究内容:

•酶的生物化学与蛋白质工程：利用定点突变与体外分子进化研究筛选新功能碱性蛋白酶、环氧化物水解酶等；

•微生物生物技术的开发与研究：利用常规的和基因工程技术改造资源微生物及其酶在相关领域方面的开发与利用。

•基因的转录调控研究：利用基因敲除、蛋白组以及转录组学等手段研究资源微生物的功能基因及其生物学的研究。

从事科研项目:

•科技部“863”专题项目《制革用碱性蛋白酶基因的分子进化研究》

•科技部支撑计划项目《西南甘薯为原料的燃料乙醇关键技术的产业化与示范》子课题

•自然科学基金《短小芽胞杆菌碱性蛋白酶基因的转录调控》

•四川省教育厅创新团队《农业废弃资源的生物转化与利用》

•横向项目《云南野生稻抗病基因资源的挖掘与利用》

从事教学工作:

•本科生教学：《基因工程》、《生物化工》

•研究生教学：《基因工程原理》、《基因表达调控》。

论文(*通讯作者):

1.Dong G, M-X He, Feng H* (2016) Functional characterization of CRISPR-Cas system in the ethanologenic bacterium Zymomonas mobilis ZM4. Adv. Microbiol. 6: 178-189.

2.Wan M-Y, Dong G, Yang B-Q, Feng H* (2016) Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate. Biotech. Lett. 38(4): 643-649.

3.Huang R, Yang Q, Feng H*. (2015) Single amino acid mutation alters thermostability of the alkaline protease from Bacillus pumilus: thermodynamics and temperature dependence. Acta Biochim. Biophys. Sin. 47(2): 98-105.

4.Shao H, Cao Q, Zhao, H, Tan X, Feng H* (2015) Construction of novel shuttle expression vectors for gene expression in Bacillus subtilis and Bacillus pumilus. J. Gen. Appl. Microbiol. 61(2): 124-131.

5.Jing Z, Feng H*(2015) Studies on the molecular docking and amino acid residues involving in recognition of substrate in proline iminopeptidase by site-directed mutagenesis. Protein J. 34(3):173-180.

6.Liu RF, Huang CL, Feng H* (2015) Salt stress represses production of extracellular proteases in Bacillus pumilus. Genet. Mol. Res. 14(2): 4339-4948

7.Zhang K, Shao H, Cao Q, He M-X, Wu B, Feng H* (2015) Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis. Appl. Microbiol. Biotechnol. 99(4), 2009-2022

8.Wu B, He M-X, Feng H, Shui Z-X, Tang X-Y, Hu Q-C, Zhang Y-Z (2014) Construction of a novel secretion expression system guided by native signal peptide of PhoD in Zymomonas mobilis. Biosci. Biotechnol Biochem. 78(4):708-713

9.冯红*,董阁,雍彬,李维 (2014) 原核CRISPR-Cas系统的结构及应用. 四川师范大学学报(自然版). 37(2):268-281

10.Yong B, Yang B-Q, Zhao C-W, Feng H* (2013) Draft genome sequence of Bacillus subtilis strain S1-4, which degrades feathers efficiently. Genome Announcements. 1(5):e00766-13. doi:10.1128/genomeA.00766-13.

11.Yong B, Yang B-Q, Feng H* (2013) Efficient degradation of raw chicken feather into soluble peptides and free amino acids by a newly isolated Bacillus subtilis S1-4. Res. J. BioTechnol. 8(9):48-55.

12.Xing K-K, Feng H* (2013) Role of amino acid residues involved in the active cavity of proline iminopeptidase in catalytic activity. Adv. Biol. Chem. 3(3):288-294.

13.Wu B, He M-X, Feng H, Zhang Y, Hu Q-C, Zhang Y-Z (2013) Construction and characterization of restriction-modification deficient mutants in Zymomonas mobilis ZM4. Chin J Appl Environ Biol. 19(2):189-197

14.Wang Z, Wang H-Y, Feng H* (2013) A simple and reproducible method for directed evolution: Combination of random mutation with dITP and DNA fragmentation with endonuclease V. Mol. Biotechnol. 53(1):49-54.

15.Zhao C-W, Wang H-Y, Zhang Y-Z, Feng H* (2012) Draft genome sequence of Bacillus pumilus BA06, a producer of alkaline serine protease with leather-dehairing function. J. Bacterial. 194(23):6668-6669.

16.Shao H-H, Cao Q-H, Tao X, Gu T-H, Chang M-J, Huang C-L, Zhang Y-Z, Feng H* (2011) Cloning and characterization of ATP synthase CF1 α gene from sweet potato. Afr. J. Biotechnol. 10(82), 19035-19042.

17.Zhang, Lin-Fen; Wu, Jin-Ming; Feng, Hong* (2011) Homology modeling and site-directed mutagenesis studies of the epoxide hydrolase from Phanerochaete chrysosporium. J. Biochem. 149(6):673-684.

18.Liang Y-J, Zhang X-Y, Luo W-J, Zhong Z-T, Lv M, Feng H, Zhao Y, Huang Q. (2011) Different multiwalled carbon nanotubes--Enzyme system and enzymatic activity. Prep. Biochem. Biotech. 2011, 41(3):243-251.

19.Li N, Wu J-M, Zhang Y-Z, Feng H* (2010) Characterization of a unique proline iminopeptidase from white-rot basidiomycetes Phanerochaete chrysosporium. Biochim. 92(7):779-788.

20.Zhang K, Feng H* (2010) Fermentation potentials of Zymomonas mobilis and its application in ethanol production from low-cost raw sweet potato. Afr. J. Biotech. 9(37):6122-6128.

21.张昆,邵欢欢,冯红* (2010) 运动发酵单胞菌利用淀粉基质生产乙醇研究进展 （阎文昭 主编）四川省科技出版社

22.杨庆军,张小炜,张义正,冯红* (2010）脱毛碱性蛋白酶定点突变的初步分析, 四川大学学报(自然版), 47(4)：936-940.

23.杨锦,冯红,万民远，焦惠科，张晓露 张义正 (2010) 短小芽孢杆菌碱性蛋白酶基因的随机突变，应用环境生物学报, 16(1):96-99.

24.He M-X, Li Y, Bai F, Liu X, Feng H, Zhang Y-Z. (2009) Ethanol production from raw sweet potato starch by mixed-culture of Paenibacillus sp. and Zymomonas mobilis. Ann. Microbiol. 59(4):749-754.

25.He M-X, Feng H, Bai F, Li Y, Liu X, Zhang Y-Z. (2009) Direct production of ethanol from raw sweet potato starch using genetically engineered Zymomonas mobilis. Afr. J. Microbiol. Res. 3(11):721-726.

26.Jiang M-F, Li X, Zhang L, Feng H, Zhang Y-Z. (2009) Gene expression analysis of Phanerochaete chrysosporium during the transition time from primary growth to secondary metabolism. J. Microbiol. 47(3):308-318.

27.Li Nian, Zhang Y-Z, Feng H* (2009) Biochemical characterization and transcriptional analysis of the epoxide hydrolase from white-rot fungus Phanerechaete chrysosporium. Acta Biochim. Biophys. Sin. 41(8):638-647.

28.Wan M-Y, Wang H-Y, Zhang Y-Z, Feng H.* (2009) Substrate specificity and thermostability of the dehairing alkaline protease from Bacillus pumilus. Appl. Biochem. Biotechnol. 159(2):394-403.

29.He M-X, Feng H, Zhang Y-Z. (2008) Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif. Biotechnol. Lett. 30:2111-2117.

30.Feng H* (2007) Mutational analysis of bacterial NAD-dependent DNA ligase: roles of motif IV in ligation catalysis. Acta Biochim. Biophys. Sin. 39(8):608-616.

31.Wu B, Hu G-K, Feng H, Wu J-M, Zhang Y-Z. (2007) Cloning and expression of an alpha-amylase gene from Phanerochaete chrysosporium. Curr. Microbiol. 55(2):105-113.

32.Feng H, Dong L, Cao W (2006) Catalytic mechanism of endonuclease V: a catalytic and regulatory two-metal model. Biochem. 45(34):10251-9.

33.Hu G-K, Feng H, Zhang T, Yan Y-H, Wu B, Jiang Q, Wu J-M, Zhang Y-Z. (2006) Molecular cloning of cDNAs for 14-3-3 and its protein interactions in a white-rot fungus Phanerochaete chrysosporium. Ann. Microbiol. 56(3):191-166.

34.胡国库, 颜永红, 姜权, 吴波, 冯红, 张义正 (2006) 黄孢原毛平革菌酵母双杂交cDNA文库的构建及应用. 应用与环境生物学报. 12(5):678-682.

35.Feng H, Dong L, Klutz AM, Aghaebrahim N, Cao W. (2005) Defining amino acid residues involved in DNA-protein interactions and revelation of 3'-exonuclease activity in endonuclease V. Biochem. 44(34):11486-95.

36.Feng H, Klutz AM, Cao W. (2005) Active site plasticity of endonuclease V from Salmonella typhimurium. Biochem. 44(2):675-83.

37.黄迎春, 冯红, 孙春昀, 陈永兵 (2005) 利用基因芯片检测性传播疾病. 四川大学学报(医学版) 36(4):576-577.

38.Feng, Hong; Parker, Jeremy M.; Lu, Jing; Cao, Weiguo (2004) Effects of deletion and site-directed mutations on ligation steps of NAD+-dependent DNA ligase: a biochemical analysis of BRCA1 C-terminal domain. Biochem. 43(39):12648-59. 被引用次数：19

39.Lu J, Tong J, Feng H, Huang J, Afonso CL, Rock DL, Barany F, Cao W. (2004) Unique ligation properties of eukaryotic NAD+-dependent DNA ligase from Melanoplus sanguinipes entomopoxvirus. Biochim. Biophys. Acta 1701(1-2):37-48.

40.黄迎春, 孙春昀, 冯红, 胡晓东, 尹海滨 (2003) 利用基因芯片检测转基因作物. 遗传. 25(3):307-310.

41.冯红, 张义正 (2000) 炭疽菌不利用硝酸盐突变体的筛选及鉴定. 微生物学通报. 27(5):341-345.

42.冯红,张义正 (2000) 黄孢原毛平革菌木质素过氧化物酶基因对营养的转录应答. 四川大学学报(自然版). 37(Sup):153-160.

43.冯红,张义正 (1999) 木质素过氧化物酶基因5′端上游调控序列的分析. 生物化学与生物物理学报. 31(6): 669-674.

Book Chapter

1.Hong Feng, Hai-Yan Wang and Yi-Zheng Zhang (2013) Endopeptidase Q. In: Neil D. Rawlings and Guy S. Salvesen, editors, Handbook of Proteolytic Enzymes. Oxford: Academic Press, pp. 3182-3188.